BabyBio column - preparation for use 

This video describes the correct way to remove the detachable end-part of the column before attaching it to the chromatographic system or whatever fitting you are using.

 

BabyBio Columns-1

To prepare the column for use:

  • Twist off the detachable end-part
  • Make sure that the detachable end-part is twisted off at the very end of the column, protecting the cone shape that fits to the chromatographic system
  • Make sure that there is a clean-cut/smooth surface on the column end after twisting
  • Make sure that the column is tightened to the chromatographic system (or fitting used)
  • Do not use excess force to tighten the column to the chromatographic system (or fitting used)
  • The above can be applied to both 1 ml and 5 ml columns.