The Purification Insights Blog

QuickTip #5: Filter everything

Posted by The Bio-Works Team on Nov 26, 2019 11:45:00 AM
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Filtering is crucial in order to protect the packed column from all solid particles in feeds or eluents. Clogging of the column will increase back-pressure and disturb the feed loading zone causing reduced resolution and it is a common problem...

Bio-Works chromatography  QuickTipsEverything needs to be filtered, every buffer every solution and solvent used, not only the feed solutions. To protect the packed column from solids always use in-line filters between the pump and the column. When an inlet frit becomes clogged it could result in a distorted flow profile, high back pressure in the column and suddenly, game over. So be sure to keep it clean by pre-filtering and regular replacement of the in-line filters.

If your feed is a bit too "cloggy" and it will take too long to filter, just do a quick centrifugation to get rid of the largest particles before starting the filtration. It will speed up the process.

Posted by The Bio-Works Team

The Bio-Works staff are seasoned and enthusiastic (yes, even a bit nerdy) experts in purification resins, methods and strategies with many years of experience of the ins and outs of this field. We love our job and we’d love to help you do yours by sharing some good solid facts, principles and best practice. In short – “purification made simple”.

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Topics: Quick Tips, Tips and Tricks, Ion exchange chromatography, IEX, Resin fouling, IMAC, Filter, SEC


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