The Purification Insights Blog

QuickTip #1: cleaning IEX columns

Posted by The Bio-Works Team on Sep 5, 2019 3:23:49 PM
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When an IEX resin has been used for some time, impurities may build up in the column causing loss of resolution or increase in back pressure (shown on the pressure monitor or seen by gap formation on the top of the column)...

Bio-Works chromatography  QuickTips Always do regular cleaning-in-place (CIP) of the resin according to the manufacture’s instructions to prolong the lifetime and resolution of the resin. But first check if the problem is actually caused by the column before starting a cleaning procedure. Disconnect one piece of equipment at a time and re-check the pressure each time. A dirty on-line filter is a common cause of increased back pressure. Check the same stage during each run, since the back pressure can vary within a run.

Posted by The Bio-Works Team

The Bio-Works staff are seasoned and enthusiastic (yes, even a bit nerdy) experts in purification resins, methods and strategies with many years of experience of the ins and outs of this field. We love our job and we’d love to help you do yours by sharing some good solid facts, principles and best practice. In short – “purification made simple”.

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Topics: Quick Tips, Tips and Tricks, Ion exchange chromatography, IEX, Cleaning in place


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