The Purification Insights Blog

How to improve process efficiency and economy in peptide production

Posted by The Bio-Works Team on May 16, 2019 11:29:13 AM

The purity requirements for therapeutic peptides are very stringent, but synthesis generates a crude peptide mixture containing failed sequences and chemical variants, while recombinant peptides have a considerable bioburden from the host cell. These crude feeds can foul the reverse phase chromatography (RPC) columns commonly used in peptide purification. Introducing an orthogonal purification step upstream significantly reduces the burden on the expensive high performance RPC column, increases the peptide yield and purity, and also prolongs column lifetime.

 

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Topics: Ion exchange chromatography, guard column, Peptides, Peptide drugs, Cleaning in place, Bioburden, RPC

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