The Purification Insights Blog

QuickTip #6: Use true buffers

Posted by The Bio-Works Team on Dec 4, 2019 9:15:00 AM

A critical and fundamental factor in both analytical and preparative chromatography is the use of true buffers. In other words, mobile phases with buffering capacity. For example, in peptide purification, there are concentrated bands of various components of the sample, including the target peptide, moving through the column and you need to have control over their tendency to dissociate...

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Topics: Quick Tips, Tips and Tricks, Protein purification, Peptides, Buffers

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