The Purification Insights Blog

QuickTip #4: yield of His-tagged proteins

Posted by The Bio-Works Team on Nov 14, 2019 9:15:00 AM

Immobilized Metal Ion Affinity Chromatography (IMAC) is used for purification of His-tagged proteins. Ni-NTA based resins are the most commonly used as the Ni2+ ion is considered to have the strongest affinity to histidine-tagged proteins but stringent requirements on the yield of target protein can make it well worth the extra effort of trying another metal ion to optimize the purification process...

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Topics: Quick Tips, Tips and Tricks, Protein purification, His-tagged, IMAC

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