The Purification Insights Blog

Improved peptide purification promises to boost process efficiency in the race to affordable insulin in China

Posted by The Bio-Works Team on May 29, 2019 9:57:45 AM

The global diabetes pandemic has created a massive demand for insulin, especially in China, which is expected to soon have half the world’s diabetes patients. Meeting this demand means developing extremely efficient production methods that can supply affordable insulin with high purity. A promising approach is to add an orthogonal step upstream the main reverse-phase chromatography (RPC) peptide purification to increase purity and also guard the expensive high-performance RPC columns from bioburden and stringent cleaning-in-place procedures.

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Topics: Biopharmaceuticals, Peptides, Peptide drugs, Cleaning in place, Resin fouling, RPC

How to improve process efficiency and economy in peptide production

Posted by The Bio-Works Team on May 16, 2019 11:29:13 AM

The purity requirements for therapeutic peptides are very stringent, but synthesis generates a crude peptide mixture containing failed sequences and chemical variants, while recombinant peptides have a considerable bioburden from the host cell. These crude feeds can foul the reverse phase chromatography (RPC) columns commonly used in peptide purification. Introducing an orthogonal purification step upstream significantly reduces the burden on the expensive high performance RPC column, increases the peptide yield and purity, and also prolongs column lifetime.

 

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Topics: Ion exchange chromatography, guard column, Peptides, Peptide drugs, Cleaning in place, Bioburden, RPC

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