The Purification Insights Blog

QuickTip #6: Use true buffers

Posted by The Bio-Works Team on Dec 4, 2019 9:15:00 AM

A critical and fundamental factor in both analytical and preparative chromatography is the use of true buffers. In other words, mobile phases with buffering capacity. For example, in peptide purification, there are concentrated bands of various components of the sample, including the target peptide, moving through the column and you need to have control over their tendency to dissociate...

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Topics: Quick Tips, Tips and Tricks, Protein purification, Peptides, Buffers

Ten things you must know when producing synthetic therapeutic peptides

Posted by The Bio-Works Team on Nov 18, 2019 9:40:39 AM

Peptide therapeutic agents are enjoying increasing success but they are challenging to produce and, not least, to purify. Purity requirements are, in fact, approaching those for small molecules. Synthesis generates a challenging crude mixture containing failed sequences and chemical variants. 

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Topics: Tips and Tricks, Biologics, Biopharmaceuticals, Peptides, Peptide drugs, orthogonal, synthetic peptides

Improved peptide purification promises to boost process efficiency in the race to affordable insulin in China

Posted by The Bio-Works Team on May 29, 2019 9:57:45 AM

The global diabetes pandemic has created a massive demand for insulin, especially in China, which is expected to soon have half the world’s diabetes patients. Meeting this demand means developing extremely efficient production methods that can supply affordable insulin with high purity. A promising approach is to add an orthogonal step upstream the main reverse-phase chromatography (RPC) peptide purification to increase purity and also guard the expensive high-performance RPC columns from bioburden and stringent cleaning-in-place procedures.

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Topics: Biopharmaceuticals, Peptides, Peptide drugs, Cleaning in place, Resin fouling, RPC

How to improve process efficiency and economy in peptide production

Posted by The Bio-Works Team on May 16, 2019 11:29:13 AM

The purity requirements for therapeutic peptides are very stringent, but synthesis generates a crude peptide mixture containing failed sequences and chemical variants, while recombinant peptides have a considerable bioburden from the host cell. These crude feeds can foul the reverse phase chromatography (RPC) columns commonly used in peptide purification. Introducing an orthogonal purification step upstream significantly reduces the burden on the expensive high performance RPC column, increases the peptide yield and purity, and also prolongs column lifetime.

 

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Topics: Ion exchange chromatography, guard column, Peptides, Peptide drugs, Cleaning in place, Bioburden, RPC

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