The Purification Insights Blog

QuickTip #6: Use true buffers

Posted by The Bio-Works Team on Dec 4, 2019 9:15:00 AM

A critical and fundamental factor in both analytical and preparative chromatography is the use of true buffers. In other words, mobile phases with buffering capacity. For example, in peptide purification, there are concentrated bands of various components of the sample, including the target peptide, moving through the column and you need to have control over their tendency to dissociate...

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Topics: Quick Tips, Tips and Tricks, Protein purification, Peptides, Buffers

QuickTip #4: yield of His-tagged proteins

Posted by The Bio-Works Team on Nov 14, 2019 9:15:00 AM

Immobilized Metal Ion Affinity Chromatography (IMAC) is used for purification of His-tagged proteins. Ni-NTA based resins are the most commonly used as the Ni2+ ion is considered to have the strongest affinity to histidine-tagged proteins but stringent requirements on the yield of target protein can make it well worth the extra effort of trying another metal ion to optimize the purification process...

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Topics: Quick Tips, Tips and Tricks, Protein purification, His-tagged, IMAC

Some general tips on protein purification strategy

Posted by The Bio-Works Team on Apr 8, 2019 4:06:46 PM

Can you generalize about something as broad as protein purification strategy? Surely it depends on the protein and the situation in general? Well, yes, of course. But there are some general aspects which might be valuable to think through before setting up any specific protein purification.

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Topics: Tips and Tricks, Protein purification



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