The Purification Insights Blog

QuickTip #3: optimizing mAb purification

Posted by The Bio-Works Team on Oct 14, 2019 1:38:24 PM

Designing an optimized purification process means maximizing yield and purity, and at the same time controlling costs. So, you remove a lot of bulk impurities during the first steps, and focus on high yield steps at the end, when every gram is precious. If you’re purifying mAbs, it would also be good to remove impurities before affinity chromatography to protect the protein A column and reduce the need for cleaning in place (CIP).

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Topics: Quick Tips, mAbs, Tips and Tricks, Ion exchange chromatography, guard column, Multimodal

QuickTip #2: choosing IEX resin

Posted by The Bio-Works Team on Sep 12, 2019 11:55:00 AM

Recovery of the biological activity of your target substance is a priority, and it’s important to check for its pH and ionic strength stability values...

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Topics: Quick Tips, Tips and Tricks, Ion exchange chromatography, IEX

QuickTip #1: cleaning IEX columns

Posted by The Bio-Works Team on Sep 5, 2019 3:23:49 PM

When an IEX resin has been used for some time, impurities may build up in the column causing loss of resolution or increase in back pressure (shown on the pressure monitor or seen by gap formation on the top of the column)...

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Topics: Quick Tips, Tips and Tricks, Ion exchange chromatography, IEX, Cleaning in place

Some general tips on protein purification strategy

Posted by The Bio-Works Team on Apr 8, 2019 4:06:46 PM

Can you generalize about something as broad as protein purification strategy? Surely it depends on the protein and the situation in general? Well, yes, of course. But there are some general aspects which might be valuable to think through before setting up any specific protein purification.

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Topics: Tips and Tricks, Protein purification

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