The Purification Insights Blog

QuickTip #10: Avoiding loss of activity of Ni charged IMAC columns

Have you experienced how Nickel-charged chromatography columns used for purifying His-tagged proteins lose their blue color, become a bit yellow and can’t bind your protein anymore? It can happen...

QuickTip #9: Purifying a His-tagged protein with low expression level

Do you find yourself having to deal with a very low yield of your target protein after the expression? Are you using a His-tag? Here are some ideas to help you with your purification.

QuickTip #8: Desalting oligos for therapeutic use

If you’re purifying therapeutic oligos you’ll need to clean the desalting resins afterwards in a far higher concentration of NaOH than the usual desalting resins will tolerate. This is how to solve...

QuickTip #7: Improve purity of viruses and vaccines

Changing from purification products based on synthetic polymers to agarose-based chromatography will increase the purity of the target virus.

QuickTip #6: Use true buffers

A critical and fundamental factor in both analytical and preparative chromatography is the use of true buffers. In other words, mobile phases with buffering capacity. For example, in peptide...

QuickTip #5: Filter everything

Filtering is crucial in order to protect the packed column from all solid particles in feeds or eluents. Clogging of the column will increase back-pressure and disturb the feed loading zone causing...

Ten things you must know when producing synthetic therapeutic peptides

Peptide therapeutic agents are enjoying increasing success but they are challenging to produce and, not least, to purify. Purity requirements are, in fact, approaching those for small molecules....

QuickTip #4: yield of His-tagged proteins

Immobilized Metal Ion Affinity Chromatography (IMAC) is used for purification of His-tagged proteins. Ni-NTA based resins are the most commonly used as the Ni2+ ion is considered to have the...

QuickTip #3: optimizing mAb purification

Designing an optimized purification process means maximizing yield and purity, and at the same time controlling costs. So, you remove a lot of bulk impurities during the first steps, and focus on...

QuickTip #2: choosing IEX resin

Recovery of your biologically active target substance is a priority, and it’s important to check for its pH and ionic strength stability values...